**New** Nanoscale 3D Imaging

The information obtained in transmission electron microscopy (TEM) is typically projected through the thickness of specimens and gives an ambiguous picture of the three-dimensional properties of nanostructures. In electron tomography, the tomographic series images are obtained at different specimen tilt-angles and are used to reconstruct the three-dimensional volume. This technique has been developed well for obtaining the information on nanoparticle morphology and distribution. Next step is to visualize nanoscale physical/chemical properties in 3D by the application of electron tomography using various signals.

A possible project is the development of dark-field scanning TEM tomography, which uses a specific diffracted beam to acquire tomographic tilt series images. This approach would be beneficial when the chemical compositions in target objects are similar to each other or crystallographic defects such as antiphase domain boundaries and dislocations are investigated. Tomography combined with other advanced TEM techniques (e.g. electron holography for electrostatic potentials) would be also an area of project interest. The project would preferably require a basic knowledge of electron microscopy and computer programming skills.

Learning Objectives:

  • Learning to use electron tomography in TEM
  • Learning 3D reconstruction/visualisation using commercial software
  • Development of novel electron tomography techniques (or implantation to Cen’s knowledge/skills)

Open to: Bachelor, Master & Special Project students

Isosurface visualization showing (a) side and (b) top views of an electron tomographic reconstruction of a GaMnAs nanowire. A GaMn nanocrystal is located on the surface of the hexagonal GaAs nanowire and is covered with a Ga droplet.


Takeshi Kasama
Senior Researcher
DTU Nanolab
+45 45 25 64 75


Elisabetta Maria Fiordaliso
+45 45 25 64 66